Fig 4.

Paf1 is required for proper transcription termination at snoRNAs. (A and B) ChIP analysis of Rpb3 occupancy in wild-type (KY1699) and paf1Δ (KY1700) cells. A no-antibody control (No Ab) was performed using wild-type (KY1699) cells. Quantification depicts average occupancy in three independent experiments as described in Materials and Methods. The SEMs are indicated by error bars, and asterisks indicate a P value of <0.05 relative to the value for the wild type. Rpb3 occupancy was examined near SNR47 (A) and SNR48 (B) at indicated locations (see Table 2 for primers used). (C and D) Quantification of Northern blot analyses of the extended SNR47 (C) and SNR48 (D) transcripts in paf1Δ (KY1700), rrp6Δ (KY1267), and paf1Δ rrp6Δ (KY2377) cells. The relative signal of rrp6Δ was set at 1, and the SEMs are indicated.