Abstract
During the assembly of the bacteriophage T4 prehead, a T4-coded protease zymogen (P21) is built into the structure. At a certain stage in head formation, the protease precursor is activated and specifically cleaves most of the prehead proteins. In this paper we show that a correlation existed between the presence of proteinaceous material in the center of the prehead core, observed by electron microscopy, and the availability of P21 during prehead assembly. In the absence of P21, the core enclosed a hold of about 35 nm long and 20 nm wide. We found the same for (i) in vitro-assembled, negatively stained prehead-like structures and (ii) in vivo-formed preheads in thin sections of T4-infected cells. We concluded that P21 was localized in the center of the prehead core.
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- Casjens S., King J. Virus assembly. Annu Rev Biochem. 1975;44:555–611. doi: 10.1146/annurev.bi.44.070175.003011. [DOI] [PubMed] [Google Scholar]
- Castillo C. J., Hsiao C. L., Coon P., Black L. W. Identification and perperties of bacteriophage T4 capsid-formation gene products. J Mol Biol. 1977 Mar 5;110(3):585–601. doi: 10.1016/s0022-2836(77)80113-7. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K., Mölbert E., Showe M., Kellenberger E. Form-determining function of the genes required for the assembly of the head of bacteriophage T4. J Mol Biol. 1970 Apr 14;49(1):99–113. doi: 10.1016/0022-2836(70)90379-7. [DOI] [PubMed] [Google Scholar]
- Müller-Salamin L., Onorato L., Showe M. K. Localization of minor protein components of the head of bacteriophage T4. J Virol. 1977 Oct;24(1):121–134. doi: 10.1128/jvi.24.1.121-134.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Onorato L., Stirmer B., Showe M. K. Isolation and characterization of bacteriophage T4 mutant preheads. J Virol. 1978 Aug;27(2):409–426. doi: 10.1128/jvi.27.2.409-426.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Showe M. K., Isobe E., Onorato L. Bacteriophage T4 prehead proteinase. I. Purification and properties of a bacteriophage enzyme which cleaves the capsid precursor proteins. J Mol Biol. 1976 Oct 15;107(1):35–54. doi: 10.1016/s0022-2836(76)80016-2. [DOI] [PubMed] [Google Scholar]
- Showe M. K., Isobe E., Onorato L. Bacteriophage T4 prehead proteinase. II. Its cleavage from the product of gene 21 and regulation in phage-infected cells. J Mol Biol. 1976 Oct 15;107(1):55–69. doi: 10.1016/s0022-2836(76)80017-4. [DOI] [PubMed] [Google Scholar]
- Wunderli H., vd Broek J., Kellenberger E. Studies related to the head-maturation pathway of bacteriophages T4 and T2:I. morphology and kinetics of intracellular particles produced by mutants in the maturation genes. J Supramol Struct. 1977;7(2):135–161. doi: 10.1002/jss.400070202. [DOI] [PubMed] [Google Scholar]
- van Driel R. Assembly of bacteriophage T4 head-related structures. IV. Isolation and association properties of T4 prehead proteins. J Mol Biol. 1980 Mar 25;138(1):27–42. doi: 10.1016/s0022-2836(80)80003-9. [DOI] [PubMed] [Google Scholar]
- van Driel R., Couture E. Assembly of bacteriophage T4 head-related strucutres. II. In vitro assembly of prehead-like structures. J Mol Biol. 1978 Aug 5;123(2):115–128. doi: 10.1016/0022-2836(78)90316-9. [DOI] [PubMed] [Google Scholar]
- van Driel R., Couture E. Assembly of the scaffolding core of bacteriophage T4 preheads. J Mol Biol. 1978 Aug 25;123(4):713–719. doi: 10.1016/0022-2836(78)90217-6. [DOI] [PubMed] [Google Scholar]