Fig 6.

Cholesterol is required for JEV infection. (A) Neuro2a and Vero cells were treated with the indicated concentrations of MβCD or filipin for 1 h and infected with JEV (MOI, 0.4) in the presence of inhibitor. At 24 hpi, cells were fixed and infection was quantified as described in Materials and Methods. (B) Neuro2a cells were treated with the indicated concentrations of MβCD or filipin for 1 h and infected with JEV (MOI, 10) in the presence of inhibitor. The endocytosed viral load was estimated by qRT-PCR of JEV positive-strand RNA. Student's t test was used to generate P values. **, P < 0.01; *, P < 0.05.