Fig 1.

Schematic presentation of the SFV4 vector carrying tissue-specific miRNA target sequences. (A) Target elements contained 6 copies of miR122 (liver-specific) or miR124 (neuron-specific) target sequences (bold and underlined) with the nsp3/nsp4 protease cleavage site sequences at both ends of the element (white on black background). (B) Element was inserted between the SFV4 nsp3 and nsp4 genes with the nsp3/nsp4 protease cleavage sites (black boxes) at both ends of miRNA target element (gray box). (C) Presentation of the whole SFV4 genome with miRNA target element location is marked with a black arrow.