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In vitro characterization of miRNA-targeted SFV4 viruses. (A) Western blot analysis of viral nsp3 and nsp4 proteins. Samples collected from BHK-21 cells infected with SFV4-miRT124, SFV4-miRT122, and SFV4 or left uninfected. (B) Viral proteins detected with Western blotting from infected Huh7 or BHK-21 cells collected at 6 h, 12 h, or 24 h postinfection. (C) The amount of viral genomic RNA measured with quantitative PCR from infected Huh7 and BHK-21 cells at 24 h postinfection.
