Fig 3.

miRNA-targeted SFV4 viruses show oncolytic potency in vitro. Five cell lines of the miR122-deficient (BHK-21, HeLa, Tera-2, and U87) or miR122-expressing cell line Huh7 were infected with SFV4, SFV4-miRT122, or SFV4-miRT124 at an MOI of 0.1. Cell survival in the infected cells was measured at 24 h postinfection (Tera-2), 48 h postinfection (Huh7, BHK-21, and HeLa), or 72 h postinfection (U87) using an ATP-based cell viability assay and plotted on the y axis as the percentage of the control values measured from uninfected cultures. The data are presented as the means of four repetitions ± standard deviations.