Fig 4.

SFV4-miRT124 shows an attenuated spread into the CNS following intraperitoneal injection in mice. (A) Survival and symptoms witnessed following intraperitoneal injection of BALB/c mice (n = 8) with 1 × 10⁶ PFU of the virus and graded as follows: 0, no symptoms; 1, ruffled fur, hunched back, weakness of limbs, and optic neuritis; 2, partial paralysis of hind limbs; 3, paralysis of hind or front limbs; 4, severe paralysis, tetraplegia; and 5, moribund/dead. (B) Virus titers measured from serum samples collected from groups of mice (n = 3) 1, 2, and 3 days postinfection (p.i.). (C) Virus titers measured from brain tissue samples collected from groups of mice (n = 3) 2, 3, and 4 days p.i. nd, not detectable.