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. 2013 Jan;87(1):304–313. doi: 10.1128/JVI.01789-12

Fig 4.

Fig 4

Rescue and in vitro passages of the shuffled chimeric viruses DS722 and DS5M3. (A) IFA confirmation using anti-PRRSV N monoclonal antibody for the rescue of the parental virus, VR2385, in MARC-145 cells infected with the supernatant of BHK-21 cells transfected with a DNA-launched PRRSV infectious clone, pIR-VR2385-CA. (B and C) IFA confirmation of the rescue of the chimeric viruses DS722 (B) and DS5M3 (C) in MARC-145 cells infected with the supernatant of BHK-21 cells transfected with shuffled clones pIR-DS722 and pIR-DS5M3, respectively. (D) There was no fluorescent signal in the MARC-145 cells infected with the supernatant of BHK-21 cells transfected with a plasmid containing a defective PRRSV VR2385 backbone.