Fig 5.

In vitro growth kinetics and plaque morphology of the shuffled chimeric viruses DS722 and DS5M3. (A) Growth kinetics of the parental virus, VR2385, and the two chimeric viruses (DS722 and DS5M3) in MARC-145 cells. The virus infectious titers (TCID₅₀/ml) were determined at the indicated time points postinfection. * and #, statistically significant difference between the chimeric viruses and the parental virus at that time point. The experiments were performed in triplicate, and the error bars indicate standard errors. (B) In vitro growth kinetics of the chimeras DS722 and DS5M3 on PAMs. The virus infectious titers (TCID₅₀/ml) were determined at the indicated time points postinfection. * and #, statistically significant difference between each of the chimeric viruses and the parental virus at that time point. The experiments were performed in triplicate, and the error bars indicate standard errors. (C) Plaque morphologies and diameters of the parental virus VR2385 and chimeric viruses DS722 and DS5M3 on MARC-145 cells.