Fig 11.

Vhs degradation of pBK2 mRNA in the presence of AMP-PNP. Rabbit reticulocyte lysates containing (lanes i through p) or lacking (lanes a through h) in vitro-translated Vhs were preincubated for 12 min in the presence (lanes e through h and m through p) or absence (lanes a through d and i through l) of 2 mM AMP-PNP, after which cap-labeled pBK2 mRNA was added and incubation continued. Aliquots were removed after 0, 3, 9, or 15 min, and the RNAs were electrophoresed through a 1.3% (wt/vol) agarose-formaldehyde gel. The relative amounts of full-length mRNAs were quantified using ImageQuant software and plotted to compare the relative decay rates of pBK2 mRNA.