Fig 4.

Prefusion conformation-destabilized F variants are reactive with MAbs recognizing triggered F structures. (A and B) Localization of the substituted residues within the structural homology model of the prefusion CDV F trimer (left and top). Each F monomer is color coded for clarification. IF was performed as described in the legend of Fig. 2B and E (right bottom). In some experiments, brief heat shocks (10 min at the indicated temperatures) were performed prior to IF. Monoclonal antibodies (MAbs) 4941 (CDV) and 186CA (MeV) were named “anti-Pre” MAbs, and MAbs 4068 (CDV) and 19GD6 (MeV) were named “anti-Trig” antibodies, due to their specificity in the binding of either the prefusion or triggered F structures, respectively.