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Pre-steady-state kinetics of nucleotide incorporation. The dependence of the reaction rate (kobs) on ATP concentration was fit to a hyperbola according to the Michaelis-Menten equation, i.e., kobs = (kpol[rNTP])/(Km + [rNTP]), where kobs is the observed reaction rate, kpol is the maximum rate of phosphodiester bond formation, and Km is the equilibrium dissociation constant. The fitted curves are overlaid for comparison of observed reaction rates of wild-type and mutant BVDV NS5Bs.
