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. 2013 Jan;87(1):199–207. doi: 10.1128/JVI.06968-11

Table 3.

Steady-state kinetic parameters for wild-type BVDV NS5B and its mutants, using poly(C)-oligo(G)12 as the substrate

Mutant Km (μM) Vmax (pmol/min/μM) kcat (min−1) kcat/Km (min−1 M−1) Ratio of (kcat/Km)WT to (kcat/Km)mutant
R283A 0.81 ± 0.015 0.32 ± 0.006 0.05 ± 0.001 64,596 3.3
R283K 0.08 ± 0.032 0.08 ± 0.003 0.01 ± 0.005 154,761 1.4
R285A 2.8 ± 0.928 0.12 ± 0.022 0.02 ± 0.003 6,749 31.3
R285K 0.14 ± 0.027 0.05 ± 0.016 0.007 ± 0.002 51,851 4.1
I287A 0.07 ± 0.026 0.01 ± 0.004 0.001 ± 0.0001 14,285 14.8
I287V 0.16 ± 0.016 0.10 ± 0.00 0.016 ± 0.004 101,265 2.1
WT 0.3 ± 0.058 30.39 ± 0.019 0.06 ± 0.003 211,401