Table 3.

Steady-state kinetic parameters for wild-type BVDV NS5B and its mutants, using poly(C)-oligo(G)₁₂ as the substrate

Mutant	Km (μM)	Vmax (pmol/min/μM)	kcat (min−1)	kcat/Km (min−1 M−1)	Ratio of (kcat/Km)WT to (kcat/Km)mutant
R283A	0.81 ± 0.015	0.32 ± 0.006	0.05 ± 0.001	64,596	3.3
R283K	0.08 ± 0.032	0.08 ± 0.003	0.01 ± 0.005	154,761	1.4
R285A	2.8 ± 0.928	0.12 ± 0.022	0.02 ± 0.003	6,749	31.3
R285K	0.14 ± 0.027	0.05 ± 0.016	0.007 ± 0.002	51,851	4.1
I287A	0.07 ± 0.026	0.01 ± 0.004	0.001 ± 0.0001	14,285	14.8
I287V	0.16 ± 0.016	0.10 ± 0.00	0.016 ± 0.004	101,265	2.1
WT	0.3 ± 0.058	30.39 ± 0.019	0.06 ± 0.003	211,401