Table 4.
Pre-steady-state kinetic parameters for single nucleotide incorporation using duplex A (14-nt–10-nt template-primer) for wild-type NS5B and its mutant derivatives
| Mutant | Km (μM)a | kpol (min−1)a | kpol/Km (min−1 M−1) |
|---|---|---|---|
| R283A | 604 ± 24 | 0.143 ± 0.002 | 236 |
| R283K | 909 ± 33 | 0.165 ± 0.027 | 181 |
| R285A | ND | ND | |
| R285K | 919 ± 27 | 0.087 ± 0.005 | 95 |
| I287A | ND | ND | |
| I287V | 614 ± 64 | 0.099 ± 0.005 | 161 |
| WT | 378 ± 82 | 0.122 ± 0.006 | 322 |
a
ND, near or at the detectable limit of NTP incorporation under the assay conditions tested.