Fig 10.

Inhibition of human, monkey, and rat complement by sCCPH and its mutants. Inhibitory activities of sCCPH and its mutants against alternative and classical pathways of human, rhesus monkey, and rat complement were measured by employing hemolytic assays using rabbit erythrocytes and antibody-coated sheep erythrocytes as complement activators, respectively. (Upper left) Correlation between IC₅₀s of sCCPH and its mutants for human and monkey alternative complement pathway (AP) (r = 0.78; P = 0.013); (upper right) correlation between IC₅₀s of sCCPH and its mutants for human and rat alternative complement pathway (r = 0.80; P = 0.009); (lower left) correlation between IC₅₀s of sCCPH and its mutants for human and monkey classical complement pathway (CP) (r = 0.84; P = 0.005); (lower right) correlation between IC₅₀s of sCCPH and its mutants for human and rat classical complement pathway (r = 0.95; P < 0.0001). The correlation coefficient (r) was calculated using the Pearson product moment correlation.