Fig 5.

HIV-1-Src-Vpx LVs pseudotyped with the SVGmu Envelope protein strictly restrict the cellular tropism of HIV-1 LVs to MDDCs. (A) MDDCs and PHA-activated PBLs were transduced at a multiplicity of infection (MOI equivalent) of 10 with HIV-1 LVs containing or not containing Src-Vpx, and they were pseudotyped with either the pantropic Env VSVg or with the DC-specific Env derived from the Sindbis virus (SVGmu). LVs were produced by transfection of 293T cells, purified, and normalized by an exo-RT activity assay against standards of known infectivity. The percentage of transduced GFP-positive cells was determined 3 days later by flow cytometry. The graph displays results (averages and SEM) obtained with cells derived from 4 different donors. *, P < 0.05 according to an unpaired Student t test. (B and C) MDDCs transduced with HIV-1-Src-Vpx LVs pseudotyped with the SVGmu envelope were further analyzed by flow cytometry for the upregulation of known cell-associated markers of MDDC maturation. (B) Representative shifts observed between mock- and vector-transduced cells (filled gray and dotted lines, respectively). (C) Averages obtained from multiple experiments. *, P < 0.05 according to an unpaired Student t test.