Fig 6.

Transduction of MDDCs with HIV-1-Src-Vpx LVs does not alter their immature phenotype or affect their ability to undergo subsequent LPS-mediated maturation. MDDCs were transduced with viral inputs yielding the same number of transduced cells between the two conditions (HIV-1 at an MOI of 5 and HIV-1-Src-Vpx at an MOI of 0.5) and examined 72 h later by flow cytometry for the analysis of the vector-based marker (A), as well as with antibodies directed against the indicated cell surface markers (B). Ab ctl, antibody control. As a control for maturation, mock (mk)-transduced MDDCs were matured with LPS for 24 h prior to analysis. The histogram plots display representative results. (C) The graph presents the normalized mean fluorescence intensities (MFI) and SEM calculated from 3 independent experiments between the indicated conditions and mock-transduced cells. Statistical analyses were performed by unpaired Student t test between each condition and mock transduction (P values of 0.05, 0.01, and 0.001 are indicated by one, two, or three asterisks, respectively). (D) Two days after transduction, cells were further incubated for 24 h with LPS to induce their maturation prior to staining with the indicated cell surface markers and flow cytometry analysis. The graph presents averages and SEM of the MFI increase observed upon LPS treatment of 3 different donors.