Fig 7.

Transduction of MDDCs with HIV-1-Src-Vpx LVs protects from transduction-mediated apoptosis. MDDCs transduced with viral inputs resulting in equivalent percentages of GFP-positive cells (MOI of 0.5 for HIV-1-Src-Vpx and of 5 for HIV-1 LVs, yielding around 20% transduction) were examined 72 h later by flow cytometry with 7AAD and annexin V. To avoid staining interference, the LV-coded GFP was substituted for a truncated hCD8 molecule. The graph presents data obtained from 4 donors in total or from transduced CD8-positive MDDCs (A and B, respectively). Statistical analyses were performed by unpaired Student t test (*, P < 0.05).