Fig 1.

Both CA and IN affect the dependence of HIV-1 infection on LEDGF/p75. (A) Schematic drawings of the parental HIV-1 (white boxes) and MLV (gray boxes) Gag and Pol proteins and the chimeric viruses. PR, protease (other abbreviations are defined in the text). Note that the MLV Gag p12 protein does not have a corresponding counterpart in HIV-1. (B) The absolute viral titers (left graph: black, E1^f/+ cells; white, E2^−/− cells) and relative viral titers of HIV-1, MLV, and the MHIV chimera in E2^−/− knockout cells expressed as a percentage of the E1^f/+ control cell infections (right graph) are shown. Data are means ± standard errors of the mean (SEM) for two independent experiments, each conducted in duplicate. (Right graph) Statistical comparison of relative titers versus WT HIV-1 (notation above bar) or between chimera viruses (brackets) was performed by paired two-tailed Student's t tests. n.s., not significant (P > 0.05); *, P < 0.05; **, P < 0.005. (C) Same as for panel B, except that the E6^f/f WT and E6^−/− knockout MEF cell pair was analyzed. In this case, the comparison of MHIV-mMA12CA to WT yielded a P value of 0.052.