Fig 6.

Binding of EV-71 and PV to an immobilized heparin-Sepharose column. EV-71 and PV supernatants were passed through a column of immobilized heparin-Sepharose and were eluted with 2 M NaCl. The titers of EV-71 and the PV vaccine strain from each fraction were quantified by TaqMan quantitative real-time PCR and plaque assays, respectively. EV-71 levels are presented as viral RNA copies per milliliter, and levels of the PV vaccine strain are presented as PFU per milliliter. Error bars represent means ± SD for each fraction.