Fig 1.

Rta enhances association of wt Z and Z(S186A) to oriLyt. (A) Chromatin immunoprecipitation (ChIP) was performed on BZKO cells transfected with empty vector (CMV), wt ZEBRA (Z), or Z(S186A) with and without Rta. The cells were treated with phosphonoacetic acid to block viral replication. Cells were collected after 48 h and cross-linked with formaldehyde. The amount of ZEBRA bound to the upstream region of oriLyt was determined by real-time PCR using the standard curve method. The AntiZ/Input ratio represents the amount of oriLyt immunoprecipitated with a specific antibody to ZEBRA relative to that present in the corresponding input sample. Fold association of ZEBRA with EBV oriLyt DNA was normalized to the AntiZ/Input ratio present in cells transfected with empty vector. (B) Bar graph demonstrating the efficiency of Z(S186A), ZEBRA+Rta, and Z(S186A)+Rta in binding to oriLyt relative to wild-type ZEBRA. The data were compiled from three independent ChIP experiments. Asterisks denote statistically significant changes (P < 0.05). ChIP of Z(S186A) to oriLyt in the presence of Rta was repeated twice, and thus no statistical analysis is available for this sample.