Fig 7.

Addition of the heterologous VP16 transactivation domain to Rta deletion mutants fails to restore the role of Rta in replication. (A) The extent of viral DNA replication was measured using real-time PCR in BZKO cells transfected with the indicated combinations of expression vectors encoding Z(S186A), a mixture of EBV replication proteins (RPs), and full-length Rta or C-terminal deletion mutants of Rta without or with VP16 fusions. (B) Protein extracts were prepared from the same group of cells. The membrane was blotted using antibodies specific to the BMRF1 early protein and the late FR3 protein.