Fig 8.

Targeting miR-K12-11 restores DUSP1 expression and reduces the secretion of promigratory factors and invasiveness for KSHV-infected endothelial cells. (A) HUVEC were incubated with purified KSHV for 2 h and were then transfected with 300 pmol of either a miR-K12-11-specific or a miR-K12-12-specific antagomir. Forty-eight hours later, immunoblotting was performed for the identification of DUSP1 protein expression. β-Actin was used as a loading control. Numbers in immunoblots indicate relative expression as determined using ImageJ software. Data are the results of one of three independent experiments. (B and C) Cells were treated as described for panel A, and culture supernatants were collected for the quantification of VEGF and IL-8 secretion by ELISA. (D) Cells were treated as described for panel A, and transwell assays were used to quantify endothelial cell invasiveness. Error bars represent the standard errors of the means for three independent experiments. *, P < 0.05; **, P < 0.01.