Fig 3.

TNPO3 sensitizes HIV-1 to inhibition by PF74. (A) Cells were inoculated with wild-type or N74D mutant HIV-GFP reporter particles in the presence of the indicated concentrations of PF74. The extent of infection was determined 48 h later by quantifying GFP-positive cells. The results were normalized to the corresponding infections performed in the absence of PF74. Normalized values are represented as means ± SEM from four independent experiments performed in duplicate. Asterisks above each point represent the difference between sh-empty and sh-TNPO3 values at that particular concentration of PF74. *, P < 0.05; **, P < 0.01. (B) Analysis of additional HIV-1 CA mutants for the effects of TNPO3 depletion on sensitivity to inhibition by PF74. Data represent means ± SEM from three independent experiments performed in duplicate.