Fig 6.

Cyclophilin A stabilizes HIV-1 cores and inhibits TNPO3-enhanced uncoating. (A) Wild-type and G89V cores were incubated at 37°C for 60 min in the presence or absence of the indicated concentrations of cyclophilin A. Following incubation, the extent of uncoating was determined. Shown are the means ± SEM from two independent experiments performed in duplicate. Asterisks above the bars indicate differences in uncoating in the absence or presence of the indicated concentration of cyclophilin A. *, P < 0.05; **, P < 0.01. (B) Uncoating of HIV-1 cores was assayed in the presence or absence of the indicated recombinant proteins (TNPO3 [1 μM] or CypA [20 μM]). Shown are the means ± SEM from three independent experiments performed in duplicate. (C and D) Cells were infected with wild-type (C and D), N74D mutant (C), or G89V mutant (D) particles, and the extent of infection was quantified by flow cytometry for GFP expression. Results are expressed as means ± SEM from three independent experiments. Asterisks indicate differences between the fold changes in infectivity in control versus sh-TNPO3 cells. *, P < 0.05.