Fig 4.

Inhibition of the arsenite-induced SG formation by the expression of JEV proteins. (A) Huh7 cells transfected with a plasmid, pCAGPM-HA-Core, were treated with or without 1.0 mM sodium arsenite for 30 min at 37°C, and the cellular localizations of G3BP and HA-Core were determined at 24 h posttransfection by immunofluorescence analysis with mouse anti-G3BP MAb and rat anti-HA MAb, followed by AF488-conjugated anti-mouse IgG and AF594-conjugated anti-rat IgG, respectively. Cell nuclei were stained with DAPI (blue). (B) Huh7 cells, which were separately transfected with a plasmid expressing an individual viral protein (pCAGPM-HA-JEV protein) as indicated in the figure, were treated with 1.0 mM sodium arsenite for 30 min at 37°C and subjected to an immunofluorescence assay using mouse anti-G3BP MAb and rat anti-HA MAb, followed by AF488-conjugated anti-mouse IgG and AF594-conjugated anti-rat IgG, respectively. Cell nuclei were stained with DAPI (blue).