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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Nat Neurosci. 2012 Nov 11;15(12):1667–1674. doi: 10.1038/nn.3256

Figure 5.

Figure 5

Spine density of NL1−/− neurons in vitro is affected by presence of neighboring NL1+/+ neurons

(a) Schematic of the co-culture experiment. NL1−/− mice were in utero electroporated to label layer 2/3 pyramidal neurons with EGFP. Dissociated cortical cultures were prepared from these mice and mixed with neurons of unlabelled WT mice at varying ratios.

(b) Representative low (left) and high (right) magnification images of neurons and spiny dendrites.

(c) Average spine densities in NL−/− neurons mixed with NL1+/+ neurons at different ratios.