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. 2013 Jan;141(1):85–94. doi: 10.1085/jgp.201210864

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© 2013 Brunet et al.

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Figure 2. — Effects of Mg²⁺_i on the β-AR stimulation of I_Ba,L. (A) Effect of 1 µM isoproterenol (Iso) on peak I_Ba,L (black traces are before isoproterenol perfusion) with 0.8 mM Mg²⁺_i. Calibration bar: 2.5 pA/pF, 50 ms. (B) Effect of isoproterenol on peak I_Ba,L with 2.4 mM Mg²⁺_i. (C) Mean I-V relationship for experiments with 0.8 mM Mg²⁺_i as described in A (n = 6). (D) Mean I-V relationship for experiments with 2.4 mM Mg²⁺_i (n = 4). (E) Effect of increased [Mg²⁺]_i on isoproterenol stimulation of peak I_Ba,L. (F) Effect of increased [Mg²⁺]_i on isoproterenol shift in the I-V relationship of I_Ba,L. The dotted lines in A and B represent zero current level. Data are presented as mean ± SEM (some errors are smaller than the symbols; *, P < 0.05).