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. 2013 Jan;141(1):85–94. doi: 10.1085/jgp.201210864

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© 2013 Brunet et al.

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Figure 3. — Effects of Mg²⁺_i on adenylate cyclase stimulation of I_Ba,L. (A) Effect of 10 µM forskolin (FSK) on peak I_Ba,L (black I_Ba,L traces are before FSK perfusion). Calibration bar: 2.5 pA/pF, 50 ms. (B) Effect of forskolin on peak I_Ba,L with 2.4 mM Mg²⁺_i. (C) Mean I-V relationship from experiments with 0.8 mM Mg²⁺_i as described in A (n = 10). (D) Mean peak stimulation of I_Ba,L with forskolin. (E) Mean I-V relationship with 2.4 mM Mg²⁺_i as in B. (F) I-V relationship from E normalized to maximum conductance values at V = 10–30 mV. The dotted lines in A and B represent zero current level. Data are presented as mean ± SEM (some errors are smaller than the symbols; *, P < 0.01).