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. 2013 Jan;141(1):85–94. doi: 10.1085/jgp.201210864

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© 2013 Brunet et al.

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Figure 6. — Effect of Mg²⁺_i on increased I_Ba,L induced by calyculin A. (A) Effect of 100 nM calyculin A on peak I_Ba,L (black trace is before calyculin A application). Calibration bar: 2.5 pA/pF, 50 ms. (B) Mean I-V relationship for experiments as described in A (control, n = 10; calyculin A, n = 16). (C) Effect of increased [Mg²⁺]_i (7.2 mM; n = 6) on the increase in peak I_Ba,L induced by calyculin A (n = 16). (D) Effect of PKA inhibition and PKC inhibition on peak I_Ba,L prestimulated by calyculin A. PKA was inhibited by the preincubation of PKA inhibitor (PKI)14–22 amide, 5 µM myristoylated peptide (n = 12). PKC was inhibited by the preincubation of PKC inhibitor 20–28 amide, 24 µM myristoylated peptide (n = 8), and 1 µM Ro 31–8220 (n = 5). Myristoyl peptide inhibitors were incubated with cells for 10 min in standard extracellular solution before beginning recordings. The dotted line in A represents zero current level. Data are presented as mean ± SEM (some errors are smaller than the symbols; *, P < 0.01).