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. 2012 Nov 15;12:87. doi: 10.1186/1472-6750-12-87

Figure 6.

Figure 6

Localization of scFv antibody in different fractions. Lane M, protein molecular weight marker ; lane L, cell lysate fraction; lane S, culture supernatant; lane P1 and P2 are, periplasmic fraction (20 μl and 10 μl respectively loaded); lane I, inclusion body (insoluble) fraction. Lane –Ve control, cell lysate of phagemid vector without scFv was used.