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. 2013 Jan 3;9(1):e1003192. doi: 10.1371/journal.pgen.1003192

Figure 3. RRIGA is primarily mediated by single-stranded annealing (SSA).

Figure 3

A) Summary of genetic requirements for the major sub-types of homologous recombination. SSA = Single Stranded Annealing; GC = Gene Conversion; BIR = Break Induced Replication. “+” = required; “−” = not required; “+/−” = required in some but not all cases; “n.d.” = not determined. B) RRIGA amplification frequencies for WT (YJL8363/8364), dnl4Δ (YJL8407/8408), rad52Δ (YJL8409/8410); rad51Δ (YJL8412/8413), rad1Δ (YJL8415/8416), msh3Δ (YJL8418/8419), and pol32Δ (YJL8421/8422) strains using the URA3 selection assay. Difference in frequency after 3 hr and 0 hr induction of re-replication was normalized for differences in the amount of re-initiation (see Text S1 and Figure S4). Data are presented as the mean ± combined error (see Text S1). C) Model for RRIGA involving SSA-mediated NAHR. Arrow, amplified segment. Triangles, non-allelic homologous sequences.