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Specific ß-galactosidase activity of the PBAD-phoP-lacZ fusion was assessed in a micA deleted strain (strain MG1452) upon overexpression of MicA or GcvB (A) or in strains deleted for micA, gcvB or both (B). Strains used in the experiment of panel B are MG1425 (wt), MG1768 (ΔgcvB), MG1430 (ΔmicA) and MG1709 (ΔmicA ΔgcvB) for the wt fusion; and MG1428 (wt) and MG1769 (ΔgcvB) for the phoPmut-lacZ fusion.
