Figure 5. Cdc13 and CST form unstable complexes with non-telomeric DNA.

(A) The sequences of the non-telomeric oligonucleotides used in the gel mobility shift assays are displayed. (B) Increasing concentrations of purified Cdc13 (0 nM, 40 nM and 80 nM) were incubated with the indicated oligonucleotide probes (7.5 nM) and the resulting complexes analyzed by gel electrophoresis and PhosphorImager scanning. (C) Labeled R1 and R3 probes (7.5 nM) were incubated with BSA (80 and 240 nM), Cdc13 (40 nM), and CST (40 nM), and the resulting complexes analyzed by gel electrophoresis and PhosphorImager scanning.