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. 2013 Jan 3;9(1):e1003101. doi: 10.1371/journal.ppat.1003101

Figure 4. Toxicity of pyocyanin is pH dependent.

Figure 4

(A, B, C) Nematode death on PGS agar with wild-type PA14, phzM, phzH, phzS, and Δphz. After bacterial growth, agar was melted and potassium phosphate pH 7 (100 mM final concentration) or equal volume of water was added. Worms were added after the agar had cooled and solidified. (D) Toxicity of exogenously added pyocyanin (10 µg/mL) in Δphz agar raised to pH 6, 7, or 8 with 100 mM potassium phosphate. Pyocyanin and buffer were added after bacterial growth.