Figure 2. The melanopic sensitivity function accounts for the impact of long wavelength conditioning stimuli on pupillary responses in rodless/coneless mice.

(A) Sensitivity of rd/rd cl pupil responses to shortwavelength (<500 nm) flash (100 ms) is not altered by 30 min pretreatment with short (498 nm) or long (644 nm) wavelength conditioning stimuli matched for melanopic illuminance (0.4 m-lux; F-test, P = 0.931). (B) Sensitivity of rd/rd cl pupil responses to <500 nm flicker (1 Hz; ON duration  = 71 ms), alternating with with short (<500 nm) or long (>600 nm) wavelength background illumination can be predicted when time-averaged irradiance is expressed in m-lux (F-test; P = 0.256).