Figure 2. Therapeutic window for MSC treatment.

Mice received 0.5×10⁶ MSCs or Veh at 3, 10 or 17 days post-HI. Because no significant difference was found between Veh groups treated at different time points we pooled all animals into one group. (A) Unimpaired forepaw preference in the CRT at 5 weeks post-insult. Quantification of ipsilateral MAP2 (B) and MBP (C) area loss measured as 1- (ipsi-/contralateral MAP2- or MBP-positive area) at 5 weeks post-insult. Insets show representative examples of MAP2 or MBP staining. Data represent mean ± SEM. Sham n = 8; Veh n = 19; MSC 3 d n = 12; MSC 10 d n = 17; MSC 17 d n = 9; *p<0.05; **p<0.01; ***p<0.001 vs. Veh. Data presented in this figure are results from pooled experiments out of 12 different litters. Treatment groups were randomly distributed between litters.