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. 2013 Jan 3;11(1):e1001455. doi: 10.1371/journal.pbio.1001455

Figure 2. Temporal activation of signaling pathways controlling translation initiation.

Figure 2

(A) Temporal expression and phosphorylation of representative proteins of key signaling pathways regulating translation initiation in mouse liver during two consecutive days. Western blots were performed on total liver extracts. Naphtol blue black staining of the membranes was used as a loading control. (B) Temporal binding of EIF4E and 4E-BP1 to 7-methyl-GTP-sepharose during two consecutive days. Total liver extracts were incubated with 7-methyl-GTP beads mimicking the mRNA cap structure. After washing of the beads, bound proteins were analyzed by Western blotting. The zeitgeber times (ZT), with ZT0, lights on; ZT12, lights off, at which the animals were sacrificed, are indicated on each panel. The lines through gels indicate where the images have been cropped.