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. 2013 Jan 3;11(1):e1001455. doi: 10.1371/journal.pbio.1001455

Figure 4. Rhythmic transcription of RP mRNA and rRNA through circadian clock regulated expression of UBF1.

Figure 4

(A) Temporal real-time RT-PCR profile of RP pre-mRNA and 45S rRNA precursor expression in mouse liver. For each time point, data are mean ± standard error of the mean (SEM) obtained from four independent animals. (B) Temporal Ubf1 mRNA (upper panel) and protein (lower panel) expression in mouse liver. mRNA were measured by real-time RT-PCR and, for each time point, data are mean ± SEM obtained from four independent animals. UBF1 protein expression was measured by Western blot on nuclear extracts during two consecutive days. The lines through gels indicate where the images have been cropped. (C–D) Temporal Ubf1 expression in mice devoid of a functional circadian clock. Ubf1 expression was measured by real-time RT-PCR with liver RNAs obtained from arrhythmic Cry1/Cry2 (C) and Bmal1 (D) KO mice and their control littermates (upper panel). Data are mean ± SEM obtained from three and two animals, respectively. Black line corresponds to the WT animals and red line to the KO. Protein levels (lower panel) were measured by Western blot on nuclear extracts. The zeitgeber times (ZT) at which the animals were sacrificed are indicated on each panel. Naphtol blue black staining of the membranes was used as a loading control.