Table 2. Primers and labeled-probes used in the nested real-time PCR assays for MPI and 6PGD genes.

Gene	Primer and probe name	Sequence (5′ – 3′)
MPI	MPI.1082.F	ACG CCC AAG TGG AAG GAT G
	MPI.1082.R	ACA CCA CTG TAC CGT TCA CC
	MPI.1082.anchor	TTC CAG ACA GAA GCC CAG CCC AAT CGT CGG – fluorescein
	MPI.1082.sensor	red670 – GTC ACG GAG GTC GTC CCG CTT CCA G
6PGD	6PGD.1262.F	CAA GGA GAT GAA GGA GGG TC
	6PGD.1262.R	CTT GTC AAC ACG TTC GTA GC
	6PGD.1262.anchor	GCC AGG GAG GCA GTC ATC ACC G – fluorescein
	6PGD.1262.sensor	red610 – AAC GAT ACA GCC GTG CTC GC