Table 7. Turnaround time and cost analysis per sample for the nested real-time PCR and MLST assays for the identification of Leishmania species from clinical specimens†.
| Procedures\assays | kDNA-PCR/MLST (USD) | Nested real-time PCR (USD) |
| kDNA-PCR | 1.3 | NAa |
| PCRb | 2.2 | 2.2 |
| Bi-directional sequencingb | 30.6 | NA |
| real-time PCRb | NA | 4.3 |
| Total cost (US dollars) | 34.1 | 6.5 |
| Turnaround time (hours) | 20 | 6 |
†
Costs represent U.S. dollar amount per individual sample based on batch analysis of 10 samples plus one positive and one negative control.
a
NA, not applicable.
b
The cost includes the analysis of both MPI and 6PGD genes per each processed sample.