Table 3. Proteins differentially expressed in the intra-cellular proteomes of controls MSCs and MSCs labeled with M-SPIO particles.
| UniProt accession No. | Protein Name | MSCs labeled with M-SPIO particles:MSCs | ||
| Experiment 1 | Experiment 2 | Experiment 3 | ||
| Significant Up-Regulated Proteins | ||||
| P30530 | Tyrosine-protein kinase receptor UFO | 1.45 | (1.40) | |
| P04114 | Apolipoprotein B-100 | (1.34) | 1.45 | |
| P35625 | Metalloproteinase inhibitor 3 | 1.38 | (1.26) | |
| P05067 | Amyloid beta A4 protein | 1.28 | (1.15) | |
| Q76M96 | Coiled-coil domain-containing protein 80 | (0.84) | 1.23 | 1.26 |
| Q13501 | Sequestosome-1 | (1.06) | 1.26 | (1.13) |
| Q14566 | DNA replication licensing factor MCM6 | (1.15) | (1.06) | 1.25 |
| P02765 | Alpha-2-HS-glycoprotein | 1.24 | (1.15) | (0.96) |
| P05121 | Plasminogen activator inhibitor 1 | (1.01) | (1.09) | 1.23 |
| P33993 | DNA replication licensing factor MCM7 | (1.07) | 1.22 | |
| P33991 | DNA replication licensing factor MCM4 | (1.05) | 1.22 | |
| Significant Down-Regulated Proteins | ||||
| P17813 | Endoglin | 0.79 | (0.93) | (0.86) |
| Q14498 | RNA-binding protein 39 | (0.85) | (0.83) | 0.77 |
| P16403 | Histone H1.2 | 0.75 | (0.71) | |
| P84103 | Splicing factor, arginine/serine-rich 3 | (0.47) | (0.71) | 0.73 |
| P16401 | Histone H1.5 | (0.76) | 0.66 | 0.61 |
| P53999 | Activated RNA polymerase II transcriptional coactivator p15 | 0.52 | 0.65 | 0.60 |
Control MSCs or MSCs labeled with M-SPIO particles were held in culture for 72 hours in Standard Media. The MSCs were subsequently collected, processed and the tryptic peptides were labeled with an isobaric tag for iTRAQ analysis using a Q-Star Elite. The experiment was repeated in triplicate. A total of 3059 proteins were identified. The fold-change values of the proteins which changed by >1.2 and <0.8 and had a p-value<0.05 in one or more experiments have been included. The fold-change values that did not meet these criteria in each experiment are depicted in brackets. A blank space indicates that a relative fold-change was not obtained for the specified protein in that replicate. For each of these proteins, the UniProt identification number, protein name, percentage of protein coverage (and number of unique peptides contributing to the sequence coverage), and fold change between the proteins in the MSCs labeled with M-SPIO particles versus control MSCs, for all replicates, are included.