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. Author manuscript; available in PMC: 2014 Jan 2.
Published in final edited form as: Anal Chem. 2012 Dec 13;85(1):138–146. doi: 10.1021/ac302256d

Figure 2.

Figure 2

Negative ion nanoelectrospray mass spectra of 10 μM myoglobin in 100 mM ammonium bicarbonate, pH 7, at electrospray potentials of (a) −0.70 kV (native) and (b) −1.4 kV (electrothermal supercharging), and in (c) methanol/water/ammonium hydroxide (48/48/4, v/v) denaturing solution at a spray potential of −0.80 kV. (d) Correlation plot of the maximum and average charge state for nine proteins obtained from denaturing solution and from native and eletrothermal supercharging from buffered aqueous solution. Data marked by “†” are discussed in the text.