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Positive ion nanoelectrospray mass spectra of 10 μM bovine serum albumin (67 kDa) in 100 mM ammonium bicarbonate, pH 7, obtained (a) using a Q-TOF mass spectrometer with high m/z capabilities, and using a Thermo LTQ™ mass spectrometer with an upper m/z limit of 4,000 at (b) +0.80 kV (native) and (c) +1.6 kV (electrothermal supercharging) spray potentials.
