Table 1.
Charge states and average charge of positive and negative ions formed in nanoESI by electrothermal supercharging from native solution and from denaturing solution.
| Protein | MW (kDa) | Charge States (+) | Average Charge (+) | Charge States (−) | Average Charge (−) | ||||
|---|---|---|---|---|---|---|---|---|---|
| Supercharged | Denatured | Supercharged | Denatured | Supercharged | Denatured | Supercharged | Denatured | ||
| Ubiquitin | 8.6 | 17 – 4+ | 14 – 4+ | 9.8 ± 0.1 | 11.0 ± 0.1 | 9 – 3− | 11 – 3− | 3.99 ± 0.03 | 6.0 ± 0.2 |
|
| |||||||||
| Barstar | 10.2 | 15 – 5+ | 12 – 5+ | 7.5 ± 0.3 | 8.8 ± 0.1 | 13 – 3− | 13 – 4− | 7.9 ± 0.3 | 9.20 ± 0.04 |
|
| |||||||||
| Cytochrome c | 12.3 | 22 – 6+ | 21 – 7+ | 14.4 ± 0.1 | 15.2 ± 0.1 | 17 – 4− | 14 – 4− | 6.6 ± 0.1 | 9.46 ± 0.02 |
|
| |||||||||
| RNase A | 13.7 | 14 – 5+ | 14 – 7+ | 9.6 ± 0.1 | 11.2 ± 0.1 | 6 – 4− | 11 – 4− | 4.98 ± 0.01 | 7.0 ± 0.1 |
|
| |||||||||
| α-Lactalbumin | 14.2 | 15 – 5+ | 13 – 6+ | 10.26 ± 0.04 | 10.38 ± 0.04 | 11 – 4− | 13 – 4− | 5.4 ± 0.1 | 8.2 ± 0.1 |
|
| |||||||||
| Lysozyme | 14.7 | 16 – 6+ | 14 – 7+ | 11.5 ± 0.5 | 10.93 ± 0.04 | 6 – 4− | 7 – 4− | 4.94 ± 0.04 | 5.47 ± 0.03 |
|
| |||||||||
| Phospholipase A2 | 15.2 | 16 – 7+ | 17 – 9+ | 10.4 ± 0.3 | 12.6 ± 0.1 | 6 – 5− | 7 – 5− | 5.4 ± 0.1 | 5.73 ± 0.03 |
|
| |||||||||
| Myoglobin | 17.6 | 28 – 7+ | 28 – 8+ | 14.9 ± 0.5 | 17.46 ± 0.02 | 20 – 5− | 20 – 5− | 12.3 ± 0.7 | 13.1 ± 0.2 |
|
| |||||||||
| EFN | 30.0 | 47 – 10+ | 42 – 10+ | 24.0 ± 0.5 | 23.5 ± 0.4 | 37 – 8− | 35 – 8− | 21.0 ± 0.2 | 22.9 ± 0.2 |