Skip to main content
. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: Dev Biol. 2012 Nov 1;373(2):373–382. doi: 10.1016/j.ydbio.2012.10.024

Figure 3. Appearance, proliferation, differentiation of Msx2-cre; KrasG12D hair follicles.

Figure 3

(A) Placode morphology and density (inset) at E15.5 revealed by keratin 17 (K17) RNA in situ hybridization in wildtype and Msx2-cre; KrasG12D (KrasG12D) embryos. Sections are counterstained with Sytox Green. Scale bar = 200 μm. (B, C) Histology of P0 and P5 hair follicles demonstrating similar hair follicle density and maturation in wildtype and KrasG12D mice. At P5, KrasG12D hair follicles begin to demonstrate abnormal orientation. Scale bars = 500 μm. (D, E) Immuno-fluorescent analysis of hair differentiation in wildtype and KrasG12D P7 hair follicles. Hair keratin (AE13)-positive staining delineates hair cortical and cuticle cells, which are present in both wildtype and KrasG12D mutant skin. Trichohyalin (AE15)-positive staining reveals internal hair medulla cells in addition to supporting IRS cells. Scale = 20 μm. (F, G) Mitotic index determined by phospho-histone H3 (ph-H3) staining on P7 wildtype and KrasG12D mutant hair follicles. Numbers of ph-H3-positive cells per hair bulb were determined for both genotypes. Scale = 20 μm.