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. 2012 Oct 27;65(1):97–104. doi: 10.1007/s10616-012-9465-y

Table 1.

Quantitative real-time PCR primer sequences

Gene Primer (forward/reverse) Significance
Mvh 5′-CGGAGAGGAACCTGAAGC-3′ Spermatogonial marker
5′-CGCCAATATCTGATGAAGC-3′
Stra8 5′-ACGACGCGTCGCTATTCCCTCTCACATCTTC-3′ Spermatogonial marker
5′-AGCGAGCTCGATGCACCTTCGACACTTG-3′
Piwill2 5′-GCACAGTCCACGTGGTGGAAA-3′ Spermatogonial marker
5′-TCCATAGTCAGGACCGGAGGG-3′
C-myc 5′-CCCTCAGTGGTCTTTCCCTAC-3 Pluripotency marker
5′-CCACAGACACCACATCAATTTC-3′
Nanog 5′-CTGGGAACGCCTCATCAA-3′ Pluripotency marker
5′-CGCATCTTCTGCTTCCTGG-3′
β Actin 5′-CTTCTTGGGTATGGAATCCTG-3′ Internal control
5′-GTGTTGGCATAGAGGTCTTTAC-3′