Fig. 2.

Characterization of 7-days cobblestone HUVECs by immuno-fluorescence. Cells (passage 1) were seeded at 20,000 cells/cm² on Aclar coated with Matrigel. When cells reached the 7-days cobblestone state, they were fixed and immuno-labeled. Nuclei were counterstained with DAPI. Cells express endothelial markers. vWF is found in discrete cytoplasmic structures and caveolin is present from the nuclear to the cell–cell contact area. Labeling for VE-cadherin (component of the adherens junctions) and claudin-5 (part of the tight junctions) is well defined at the cell periphery as expected for a tight cobblestone monolayer. Asterisks mark the same cell. Scale bar (applicable to all the panels): 30 μm