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Effect of AlaGln on 12C-5 cell line in 1 L bioreactor culture. a Comparison of cell growth. Combination of the supplements in basal and feed media was (1) starting culture with Gln and feeding medium with Gln (black circle) and (2) starting culture with AlaGln and feeding medium with AlaGln (black triangle). b Comparison of MAb titer at the end of cell culture. c Comparison of SPR. Three other independent experiments with similar conditions exhibited similar results
