Fig. 6.

a Time course change of ammonia during cell culture with 1 L bioreactor of 1A7-15 (1) starting culture with Gln and feeding medium with Gln (black circle), (2) starting culture with Gln and feeding medium with AlaGln (black square) and (3) starting culture with AlaGln and feeding medium with AlaGln (black triangle). b Time course change of ammonia in culture medium including either 12 mmol/L Gln (black circle) or 12 mmol/L AlaGln (black square). c Ammonia effect on cell culture with Erlenmeyer flask of 1A7-15: ammonia 0 mmol/L (black circle), ammonia 10 mmol/L (black square), ammonia 20 mmol/L (black triangle), ammonia 30 mmol/L (plus symbols), ammonia 40 mmol/L (times symbols), ammonia 50 mmol/L (circle), ammonia 60 mmol/L (asterisk). d Specific cell growth rate of c between days 0 and 4. e Ammonia effect on 1A7-15 cell growth in 1 L bioreactor culture: Gln–Gln (black circle), AlaGln–AlaGln (black triangle) and Gln–Gln + 24 mmol/L ammonia (circle). f Comparison of MAb titer at the end of cell culture (e). Two other independent experiments with similar conditions exhibited similar results